[Histonet] light staining

[Cynthia Pyse]

Check your decolorizer if you are using one.

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Nancy
Schmitt
Sent: Tuesday, February 09, 2010 1:38 PM
To: Histonet (histonet <@t> lists.utsouthwestern.edu)
Subject: [Histonet] light staining

Hello Histonetters

We are having trouble with light staining from the hematoxylin.  It is the
same lot number we have been in.  We changed it out and still the same.  All
reagent containers were emptied, cleaned and refilled yesterday.  We have
not changed anything with the timings on our stainer.  What am I missing?

Thanks for your help
Nancy Schmitt
United Clinical Laboratories
Dubuque, IA




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