[Histonet] light staining

[Tony Henwood]

I would have a look at the haematoxylin stained section before you
differentiate.

Does it look OK? If it does then do not differentiate, blue only. (also
someone has not accidently put acid in the blueing solution? Check with
apH papers). You might try extending the Hx time.
If it still looks pale after the eosin, then the acid in the eosin is
continuing the differentiation process. Extend your Hx staining time &
decrease the eosin time.

If it is a young Hx then you might find that the staining improves with
time (ie it "ripens").


Regards

Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC)
Laboratory Manager & Senior Scientist
Tel: 612 9845 3306
Fax: 612 9845 3318
the children's hospital at westmead 
Cnr Hawkesbury Road and Hainsworth Street, Westmead 
Locked Bag 4001, Westmead NSW 2145, AUSTRALIA 




-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Nancy
Schmitt
Sent: Wednesday, 10 February 2010 5:38 AM
To: Histonet (histonet <@t> lists.utsouthwestern.edu)
Subject: [Histonet] light staining


Hello Histonetters

We are having trouble with light staining from the hematoxylin.  It is
the same lot number we have been in.  We changed it out and still the
same.  All reagent containers were emptied, cleaned and refilled
yesterday.  We have not changed anything with the timings on our
stainer.  What am I missing?

Thanks for your help
Nancy Schmitt
United Clinical Laboratories
Dubuque, IA




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