[Histonet] light staining

V. Neubert histonet.nospam <@t> vneubert.com
Tue Feb 9 12:50:24 CST 2010


Check pH of your washing water.
pH too low will result in not staining at all.

I'd clean the stainer as well.


> Hello Histonetters
>
> We are having trouble with light staining from the hematoxylin.  It is the same lot number we have been in.  We changed it out and still the same.  All reagent containers were emptied, cleaned and refilled yesterday.  We have not changed anything with the timings on our stainer.  What am I missing?
>
> Thanks for your help
> Nancy Schmitt
> United Clinical Laboratories
> Dubuque, IA
>
>
>
>
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