[Histonet] Re: Cryoprotection

[sgoebel <@t> xbiotech.com]

   I assumed distilled water?

   Sarah Goebel, B.A., HT (ASCP)

   Histotechnician
   
   XBiotech USA Inc.

   8201 E
   Austin, Texas  78744

   (512)386-5107

   -------- Original Message --------
   Subject: RE: [Histonet] Re: Cryoprotection
   From: "Mejia, Maria" <[1]Maria.Meji   Date: Tue, August 24, 2010 10:31 am
   To: vaigunda ragavendran <[2]rags_j   "[3]histonet <@t> lists.utsout   histonet <@t> lists.utsouthwestern.edu>
   Can  you  be more specific & tell us what DW means & please give us t   he protocol for the sucrose including
   the amount of DW used. This way we can help you.
   Regards
   Maria Mejia
   Histology Supervisor
   Department of Neurosurgery
   UCSF
   San Francisco, CA
   ________________________________________
   From:          [5]histonet   [[6]histonet-bounces <@t> lists.utsouthwestern.edu] On    ragavendran [[7]rag   Sent: Friday, August 20, 2010 1:19 PM
   To: [8]histonet <@t> lists.uts   Subject: [Histonet] Re: Cryoprotection
   Hii,
   If  instead  of  sucrose in PBS, we use sucrose in DW by mistake, what
   might h   Please lemme know..
   Rags,
   Postdoctoral Research Fellow,
   Verge Lab,
   Department of Anatomy and Cell Biology,
   Room No: 5800, Saskatoon City Hospital,
   701, Queen Street,
   Saskatoon, Saskatchewan S7K 0M7,
   Canada.
   Phone (Lab): +1 306-655-8714
   (Facsimile): +1 306-655-8709
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References

   1. 3D"mailto:Maria.Mejia <@t> ucsf.edu"
   2. 3D"mailto:rags_jeg <@t> yahoo.co.in"
   3. 3D"mailto:histonet <@t> lists.utsouthwestern.edu"
   4. 3D"mailto:histonet <@t> lists.utsouthwestern.edu"
   5. 3D"mailto:histonet-bounces <@t> lists.utsouthwestern.edu"
   6. 3D"mailto:histonet-bounces <@t> l   7. 3D"mailto:rags_jeg <@t> yahoo.co.in"
   8. 3D"mailto:histonet <@t> lists.utsouthwestern.edu"
   9. 3D"mailto:Histonet <@t> lists.utsouthwestern.edu"
  10. 3D"http://lists.utsouthwestern.edu/mailman/listinfo/histonet"
  11. 3D"mailto:Histonet <@t> lists.utsouthwestern.edu"
  12. 3D"http://lists.utsouthwestern.edu/mailman/listinfo/histonet"