[Histonet] Re: Cryoprotection

[vaigunda ragavendran]

Yes, I meant distilled water.. but now there is no problem.. I just checked my cut sections that were mistakenly cryoprotected in sucrose/DW instead of in sucrose/PBS and there is not a significant degree of cell lysis.. the dorsal root ganglion neurons are mostly intact and not lysed.. I felt happy yest and decided to be more careful the next time I do cryoprotection of my tissues..

Anyway thanks Sarah for your comment..

Byee..

Rags,
Postdoctoral Research Fellow,
Verge Lab,
Department of Anatomy and Cell Biology,
Room No: 5800, Saskatoon City Hospital,
701, Queen Street,
Saskatoon, Saskatchewan S7K 0M7,
Canada.

Phone (Lab): +1 306-655-8714
          (Facsimile): +1 306-655-8709


--- On Wed, 25/8/10, sgoebel <@t> xbiotech.com <sgoebel <@t> xbiotech.com> wrote:

From: sgoebel <@t> xbiotech.com <sgoebel <@t> xbiotech.com>
Subject: RE: [Histonet] Re: Cryoprotection
To: "Mejia,Maria" <Maria.Mejia <@t> ucsf.edu>
Cc: "vaigunda ragavendran" <rags_jeg <@t> yahoo.co.in>, "histonet <@t> lists.utsouthwestern.edu" <histonet <@t> lists.utsouthwestern.edu>
Date: Wednesday, 25 August, 2010, 12:53 AM

I assumed distilled water?

Sarah Goebel, B.A., HT (ASCP)Histotechnician
XBiotech USA Inc.8201 East Riverside Dr. Bldg 4 Suite 100Austin, Texas  78744(512)386-5107





-------- Original Message --------

Subject: RE: [Histonet] Re: Cryoprotection

From: "Mejia, Maria" <Maria.Mejia <@t> ucsf.edu>

Date: Tue, August 24, 2010 10:31 am

To: vaigunda ragavendran <rags_jeg <@t> yahoo.co.in>,

"histonet <@t> lists.utsouthwestern.edu" <histonet <@t> lists.utsouthwestern.edu>



Can you be more specific & tell us what DW means & please give us the protocol for the sucrose including

the amount of DW used.  This way we can help you.



Regards



Maria Mejia

Histology Supervisor

Department of Neurosurgery

UCSF

San Francisco, CA

________________________________________

From: histonet-bounces <@t> lists.utsouthwestern.edu [histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of vaigunda ragavendran [rags_jeg <@t> yahoo.co.in]

Sent: Friday, August 20, 2010 1:19 PM

To: histonet <@t> lists.utsouthwestern.edu

Subject: [Histonet] Re: Cryoprotection



Hii,



If instead of sucrose in PBS, we use sucrose in DW by mistake, what might happen to fixed tissue sections during cryoprotection..



Please lemme know..



Rags,

Postdoctoral Research Fellow,

Verge Lab,

Department of Anatomy and Cell Biology,

Room No: 5800, Saskatoon City Hospital,

701, Queen Street,

Saskatoon, Saskatchewan S7K 0M7,

Canada.



Phone (Lab): +1 306-655-8714

          (Facsimile): +1 306-655-8709







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