[Histonet] Test for cell viability in paraffin section?

[annigyg <@t> gmail.com]

Hey Ruel
Once we are done with them and they are all nicely embedded in paraffin wax...the cells are not viable...they are DEAD
Annieinarabia
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-----Original Message-----
From: "Reuel Cornelia" <Reuel.Cornelia <@t> tsrh.org>
Sender: histonet-bounces <@t> lists.utsouthwestern.edu
Date: Wed, 18 Aug 2010 08:55:48 
To: <histonet <@t> lists.utsouthwestern.edu>
Subject: [Histonet] Test for cell viability in paraffin section?

Would you know a  techniqe to confirm cell viability in paraffin embedded tissue?  Propidium and methylene blue staining are used in cell culturing to detect cell viability.  Is there similar technique for detecting cell viability in tissue?
This question was asked by our research director, Can you please share your opinion on this and if there is chemical test to detect vaibility of cells in a paraffin tissue?

Reuel Cornelia, BS MT, AMT
Cellular Pathology
Texas Scottish Rite Hospital for Children
2222 Welborn Street
Dallas, TX 75219
Tel: 214-559-7766
fax: 214-559-7768



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