[Histonet] Test for cell viability in paraffin section?

Edwards, Richard E. ree3 <@t> leicester.ac.uk
Wed Aug 18 09:39:17 CDT 2010

Cell/tissue  embedded in paraffin wax  are  as  dead as  the  famous Norwegian Blue parrot, however  you could  demonstrate  programmed cell death, apoptosis.

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Reuel Cornelia
Sent: 18 August 2010 14:56
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Test for cell viability in paraffin section?

Would you know a  techniqe to confirm cell viability in paraffin embedded tissue?  Propidium and methylene blue staining are used in cell culturing to detect cell viability.  Is there similar technique for detecting cell viability in tissue?
This question was asked by our research director, Can you please share your opinion on this and if there is chemical test to detect vaibility of cells in a paraffin tissue?

Reuel Cornelia, BS MT, AMT
Cellular Pathology
Texas Scottish Rite Hospital for Children
2222 Welborn Street
Dallas, TX 75219
Tel: 214-559-7766
fax: 214-559-7768

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