[Histonet] Test for cell viability in paraffin section?
Edwards, Richard E.
ree3 <@t> leicester.ac.uk
Wed Aug 18 09:39:17 CDT 2010
Cell/tissue embedded in paraffin wax are as dead as the famous Norwegian Blue parrot, however you could demonstrate programmed cell death, apoptosis.
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Reuel Cornelia
Sent: 18 August 2010 14:56
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Test for cell viability in paraffin section?
Would you know a techniqe to confirm cell viability in paraffin embedded tissue? Propidium and methylene blue staining are used in cell culturing to detect cell viability. Is there similar technique for detecting cell viability in tissue?
This question was asked by our research director, Can you please share your opinion on this and if there is chemical test to detect vaibility of cells in a paraffin tissue?
Reuel Cornelia, BS MT, AMT
Texas Scottish Rite Hospital for Children
2222 Welborn Street
Dallas, TX 75219
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