[Histonet] Test for cell viability in paraffin section?

Rene J Buesa rjbuesa <@t> yahoo.com
Wed Aug 18 09:06:39 CDT 2010

After a "deadly" fixation, followed by a complete dehydration, clearing and paraffin wax infiltration, the only potentially surviving "entities" are prions.
No cell will be viable after this treatment and if you get some positive results of viability with what ever test you use, I caution to believe those results.
Your research director evidently is absolutely ignorant about tissue processing!
René J.

--- On Wed, 8/18/10, Reuel Cornelia <Reuel.Cornelia <@t> tsrh.org> wrote:

From: Reuel Cornelia <Reuel.Cornelia <@t> tsrh.org>
Subject: [Histonet] Test for cell viability in paraffin section?
To: histonet <@t> lists.utsouthwestern.edu
Date: Wednesday, August 18, 2010, 9:55 AM

Would you know a  techniqe to confirm cell viability in paraffin embedded tissue?  Propidium and methylene blue staining are used in cell culturing to detect cell viability.  Is there similar technique for detecting cell viability in tissue?
This question was asked by our research director, Can you please share your opinion on this and if there is chemical test to detect vaibility of cells in a paraffin tissue?

Reuel Cornelia, BS MT, AMT
Cellular Pathology
Texas Scottish Rite Hospital for Children
2222 Welborn Street
Dallas, TX 75219
Tel: 214-559-7766
fax: 214-559-7768

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