[Histonet] Re: muscle stain

[Andrea Marion]

There is an immunofluorescence technique that uses wheat germ agglutinin
(WGA) conjugated to a fluorophore (usually FITC). WGA binds only to the
cell periphery, leaving you with beautiful traces of the outside of each
cell. The cell area is then simple to analyze with ImageJ or any other
software program. It's often used to measure cardiomyocyte size in
hypertrophy studies. I can provide a protocol if requested.

If you have access to this article, there is a detailed description of the
technique and great images: Regional changes in myocyte structure in model
of canine right atrial hypertrophy. Am J Physiol Heart Circ Physiol 267:
H1279-H1287, 1994. P. C. Dolber, R. P. Bauman, J. C. Rembert and J. C.
Greenfield Jr.

If not, here is a freely accessible publication. Figure 9, panel B shows
what the result looks like. Cardiomyocyte GATA4 functions as a
stress-responsive regulator of angiogenesis in the murine heart. J Clin
Invest. 2007; 117(11):3198.
http://www.jci.org/articles/view/32573/figure/9

Andrea Marion
Graduate Student
University of Illinois - Chicago
amario3 <at> uic <dot> edu

> To: histonet <@t> lists.utsouthwestern.edu
> Subject: [Histonet] muscle stain
>
> I am posting this for a friend.  . They would like to stain muscle.
> The eosin and hematoxylin stain gives us too much information since it
> stains
> the nucleus separately from the cytoplasm and confuses the image analysis
> software. What we need is the measurement of  the cell diameter only, a
> simpler
> stain. Thanks for any info you can give me. Deon Simon
> Any help you can give her on how to measure or a stain will be very much
> appreciated.
>
> Margaret Perry HT(ASCP)
> Dept of Veterinary and  Biomedical services
> Box 2175
> South Dakota State University
> Brookings SD 57007
> 605-688-5638
>