[Histonet] Re: muscle stain

[Merced M Leiker]

Andrea - Wow this looks great. We do a lot of cardiomyocyte staining and 
studying and this could be helpful. Thanks for sharing!

Merced M Leiker
Research Technician III
Cardiovascular Medicine
348 Biomedical Research Building
State University of New York at Buffalo
3435 Main St, Buffalo, NY 14214  USA
leiker <@t> buffalo.edu
716-829-6118 (Ph)
716-829-2665 (Fx)

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--On Wednesday, August 18, 2010 8:18 AM -0500 Andrea Marion 
<amario3 <@t> uic.edu> wrote:

> There is an immunofluorescence technique that uses wheat germ agglutinin
> (WGA) conjugated to a fluorophore (usually FITC). WGA binds only to the
> cell periphery, leaving you with beautiful traces of the outside of each
> cell. The cell area is then simple to analyze with ImageJ or any other
> software program. It's often used to measure cardiomyocyte size in
> hypertrophy studies. I can provide a protocol if requested.
>
> If you have access to this article, there is a detailed description of the
> technique and great images: Regional changes in myocyte structure in model
> of canine right atrial hypertrophy. Am J Physiol Heart Circ Physiol 267:
> H1279-H1287, 1994. P. C. Dolber, R. P. Bauman, J. C. Rembert and J. C.
> Greenfield Jr.
>
> If not, here is a freely accessible publication. Figure 9, panel B shows
> what the result looks like. Cardiomyocyte GATA4 functions as a
> stress-responsive regulator of angiogenesis in the murine heart. J Clin
> Invest. 2007; 117(11):3198.
> http://www.jci.org/articles/view/32573/figure/9
>
> Andrea Marion
> Graduate Student
> University of Illinois - Chicago
> amario3 <at> uic <dot> edu
>
>> To: histonet <@t> lists.utsouthwestern.edu
>> Subject: [Histonet] muscle stain
>>
>> I am posting this for a friend.  . They would like to stain muscle.
>> The eosin and hematoxylin stain gives us too much information since it
>> stains
>> the nucleus separately from the cytoplasm and confuses the image analysis
>> software. What we need is the measurement of  the cell diameter only, a
>> simpler
>> stain. Thanks for any info you can give me. Deon Simon
>> Any help you can give her on how to measure or a stain will be very much
>> appreciated.
>>
>> Margaret Perry HT(ASCP)
>> Dept of Veterinary and  Biomedical services
>> Box 2175
>> South Dakota State University
>> Brookings SD 57007
>> 605-688-5638
>>
>
>
>
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Merced M Leiker
Research Technician III
Cardiovascular Medicine
348 Biomedical Research Building
State University of New York at Buffalo
3435 Main St, Buffalo, NY 14214  USA
leiker <@t> buffalo.edu
716-829-6118 (Ph)
716-829-2665 (Fx)

No trees were harmed in the sending of this email.
However, many electrons were severely inconvenienced.