[Histonet] muscle stain

Birgitta Stephenson bstephen <@t> fastmail.fm
Wed Aug 18 01:57:52 CDT 2010


Toluidine blue stains striated muscle nicely.

Regards
Birgitta Stephenson University of Queensland Microscopy Research Lab
On Tue, 17 Aug 2010 17:18:18 -0400, "Sherwood, Margaret "
<MSHERWOOD <@t> PARTNERS.ORG> said:
> Masson Trichrome stains collagen and muscle.
> 
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu
> [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Perry,
> Margaret
> Sent: Tuesday, August 17, 2010 5:06 PM
> To: histonet <@t> lists.utsouthwestern.edu
> Subject: [Histonet] muscle stain
> 
> I am posting this for a friend.  . They would like to stain muscle.
> The eosin and hematoxylin stain gives us too much information since it
> stains
> the nucleus separately from the cytoplasm and confuses the image analysis
> software. What we need is the measurement of  the cell diameter only, a
> simpler
> stain. Thanks for any info you can give me. Deon Simon
> Any help you can give her on how to measure or a stain will be very much
> appreciated.
> 
> Margaret Perry HT(ASCP)
> Dept of Veterinary and  Biomedical services
> Box 2175
> South Dakota State University
> Brookings SD 57007
> 605-688-5638
> 
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-- 
  Birgitta Stephenson
  bstephen <@t> fastmail.fm

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