[Histonet] please enter

[Houston, Ronald]

Mohamed,



Deposition of C4d is an inactive fragment of C4b (derived from complement component C4). It provides in-situ evidence of complement activation following alloantibody binding to graft vasculature.



As such, the development of antibodies against C4d has dramatically improved the ability to diagnose antibody mediated rejection (AMR).



We use the C4d (along with C3d) antibody from Cell Marque to investigate heart, lung and kidney biopsies for antibody mediated rejection in transplanted organs - in fact many consider C4d immunohistochemistry to be the gold standard for evaluation of AMR.



C4d has also been postulated as a specific marker in the differential diagnosis of Follicular Lymphoma vs MALT lymphoma (Pathology - Research and Practice 2007; 203:163-167)


Ronnie Houston, MS HT(ASCP)QIHC
Anatomic Pathology Manager

ChildLab, a Division of Nationwide Children's Hospital

www.childlab.com


700 Children's Drive
Columbus, OH 43205
(P) 614-722-5450
(F) 614-722-2899
ronald.houston <@t> nationwidechildrens.org<mailto:ronald.houston <@t> nationwidechildrens.org>
www.NationwideChildrens.org<http://www.NationwideChildrens.org>




-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of mohamed abd el razik
Sent: Friday, August 13, 2010 1:46 PM
To: Histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] please enter



Dear histonetters

i have a requist for all of you as i'm new in histochemistery and IHC as many other frinds i know in histonet . we found it difficult to know the name and use of many antibodies in many many topics so we can't understand the all masages and replaing on it. and lose the opportunity to learn from it!! and we are  ashamed  to talk about that with experts like you.but the group lose its functionality in teaching IHC for the new histologiest sector.

so i put our proplem on your hands and my hope is to help us to find solution



an example of what i'm taking about is what is C4d and for what it is used?



thanx in advance to your help and for these amazing group



mohamed

Faculty of Vet. Med.

Cairo Univ. - Egypt







We use the same antibody from Cell Marque on the Dako Autostainers with great success.



Loralee McMahon, HTL (ASCP)

Immunohistochemistry Supervisor

Strong Memorial Hospital

Department of Surgical Pathology

(585) 275-7210

________________________________________

From: histonet-bounces <@t> lists.utsouthwestern.edu [histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Houston, Ronald [Ronald.Houston <@t> nationwidechildrens.org]

Sent: Friday, August 13, 2010 10:29 AM

To: 'Martha Ward'; histonet <@t> lists.utsouthwestern.edu

Subject: [Histonet] RE: C4d on paraffin sections



We use the C4d from Cell Marque now with great results; it is an IVD.



ER1 20 minutes on the BondMax and Bond III





Ronnie Houston

Anatomic Pathology Manager

Nationwide Children's Hospital

Columbus OH 43205

(614) 722 5450

-----Original Message-----

From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Martha Ward

Sent: Friday, August 13, 2010 9:46 AM

To: histonet <@t> lists.utsouthwestern.edu

Subject: [Histonet] C4d on paraffin sections



I need some advice.  We have been trying to do C4d on paraffin sections

without much success.  Does anyone have a good protocol we could try?

We use the C4d from Quidel for our frozen sections and ideally I would

like a procedure that we can use with our Bond Max, but I may be hoping

for too much!  Thanks in advance for your help.



Martha Ward, MT (ASCP) QIHC

Assistant Manager, Molecular Diagnostics Lab

Wake Forest University Baptist Medical Center

336-716-2104







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