[Histonet] please enter

[mohamed abd el razik]

Dear histonetters
i have a requist for all of you as i'm new in histochemistery and IHC as many other frinds i know in histonet . we found it difficult to know the name and use of many antibodies in many many topics so we can't understand the all masages and replaing on it. and lose the opportunity to learn from it!! and we are  ashamed  to talk about that with experts like you.but the group lose its functionality in teaching IHC for the new histologiest sector.
so i put our proplem on your hands and my hope is to help us to find solution 
 
an example of what i'm taking about is what is C4d and for what it is used?
 
thanx in advance to your help and for these amazing group
 
mohamed
Faculty of Vet. Med.
Cairo Univ. - Egypt
 
 
 
We use the same antibody from Cell Marque on the Dako Autostainers with great success. 

Loralee McMahon, HTL (ASCP)
Immunohistochemistry Supervisor
Strong Memorial Hospital
Department of Surgical Pathology
(585) 275-7210
________________________________________
From: histonet-bounces <@t> lists.utsouthwestern.edu [histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Houston, Ronald [Ronald.Houston <@t> nationwidechildrens.org]
Sent: Friday, August 13, 2010 10:29 AM
To: 'Martha Ward'; histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] RE: C4d on paraffin sections

We use the C4d from Cell Marque now with great results; it is an IVD.

ER1 20 minutes on the BondMax and Bond III


Ronnie Houston
Anatomic Pathology Manager
Nationwide Children's Hospital
Columbus OH 43205
(614) 722 5450
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Martha Ward
Sent: Friday, August 13, 2010 9:46 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] C4d on paraffin sections

I need some advice.  We have been trying to do C4d on paraffin sections
without much success.  Does anyone have a good protocol we could try?
We use the C4d from Quidel for our frozen sections and ideally I would
like a procedure that we can use with our Bond Max, but I may be hoping
for too much!  Thanks in advance for your help.

Martha Ward, MT (ASCP) QIHC
Assistant Manager, Molecular Diagnostics Lab
Wake Forest University Baptist Medical Center
336-716-2104



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