[Histonet] please enter

Sun Aug 15 18:11:58 CDT 2010

C4d Immunoreactivity has been used to assess transplant failure in kidneys and hearts

Peritubular capillary deposition of C4d has been shown to be associated with both acute humoral and vascular rejection and increased graft loss (7,8). This was found to be independent of histological rejection type (7).

Patients were considered C4d positive if 25% of the peritubular capillaries exhibited circumferential staining (7). Successful staining relies on Microwave antigen retrieval.

Peritubular capillary C4d deposition in acute allograft rejection is a predictor of long term graft failure (7) and it has been suggested that these patients would benefit from intensive therapy, potentially preventing the previously reported high graft failure rate (8).

7.	Herzenberg, A.M., Gill, J.S., Djurdjev, O., Magil, A.B., (2002) "C4d deposition in acute rejection: An Independent Long-term Prognostic factor" J Am Soc Nephrol 13(1):234-41.
8.	Nickeleit, V., Zeeiler, M., Gudat, F., Theil, G., Mihatsch, M.J., (2002) "Detection of the complement degradation product C4d in renal allografts: diagnostic and therapeutic implications: J Am Soc Nephrol 13(1):242-51

Regards

Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC)
Laboratory Manager & Senior Scientist
Tel: 612 9845 3306
Fax: 612 9845 3318
the children's hospital at westmead 
Cnr Hawkesbury Road and Hainsworth Street, Westmead 
Locked Bag 4001, Westmead NSW 2145, AUSTRALIA 

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of mohamed abd el razik
Sent: Saturday, 14 August 2010 3:46 AM
To: Histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] please enter

Dear histonetters
i have a requist for all of you as i'm new in histochemistery and IHC as many other frinds i know in histonet . we found it difficult to know the name and use of many antibodies in many many topics so we can't understand the all masages and replaing on it. and lose the opportunity to learn from it!! and we are  ashamed  to talk about that with experts like you.but the group lose its functionality in teaching IHC for the new histologiest sector. so i put our proplem on your hands and my hope is to help us to find solution 

an example of what i'm taking about is what is C4d and for what it is used?

thanx in advance to your help and for these amazing group

mohamed
Faculty of Vet. Med.
Cairo Univ. - Egypt

We use the same antibody from Cell Marque on the Dako Autostainers with great success. 

Loralee McMahon, HTL (ASCP)
Immunohistochemistry Supervisor
Strong Memorial Hospital
Department of Surgical Pathology
(585) 275-7210
________________________________________
From: histonet-bounces <@t> lists.utsouthwestern.edu [histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Houston, Ronald [Ronald.Houston <@t> nationwidechildrens.org]
Sent: Friday, August 13, 2010 10:29 AM
To: 'Martha Ward'; histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] RE: C4d on paraffin sections

We use the C4d from Cell Marque now with great results; it is an IVD.

ER1 20 minutes on the BondMax and Bond III

Ronnie Houston
Anatomic Pathology Manager
Nationwide Children's Hospital
Columbus OH 43205
(614) 722 5450
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Martha Ward
Sent: Friday, August 13, 2010 9:46 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] C4d on paraffin sections

I need some advice.  We have been trying to do C4d on paraffin sections without much success.  Does anyone have a good protocol we could try? We use the C4d from Quidel for our frozen sections and ideally I would like a procedure that we can use with our Bond Max, but I may be hoping for too much!  Thanks in advance for your help.

Martha Ward, MT (ASCP) QIHC
Assistant Manager, Molecular Diagnostics Lab
Wake Forest University Baptist Medical Center
336-716-2104

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