[Histonet] Re: [Special Stains] Masson's Trichrome

Rena Fail renafail <@t> bellsouth.net
Mon Aug 9 10:24:01 CDT 2010

It depends on the number of slides as well as time on the shelf and a little 
common sense.Your stock solutions have a longer shelf life than your working.  I 
worked in a lab that perfomed a large number of Masson's by hand( until 
purchasing the Artisan)  We changed the solutions once a week to insure 
consistent staining.  If you perform these stains only twice a month, your 
results will be more consistant if you make the Weigert's fresh  and watch the 
level of your aniline blue, evaporation will make it more concentrated.  On the 
other hand a large volume of slides will weaken the working solutions. following 
is a link that lists some guidelines for storing stock solutions

Rena Fail


----- Original Message ----
From: "Sherwood, Margaret" <MSHERWOOD <@t> PARTNERS.ORG>
To: histonet <@t> lists.utsouthwestern.edu
Sent: Mon, August 9, 2010 10:32:48 AM
Subject: [Histonet] Re: [Special Stains] Masson's Trichrome

To all:

We keep having the same discussion re:  special stains.  How long can you use
them before discarding?  I am specifically referring to the stains used in
Masson's Trichrome.

We are a research lab and, therefore, don't run the volume that most labs do.
We have found, for instance, that we can use the Weigert's Hematoxylin (A&B)
more than once, with filtering (even though it is stated it should be made
fresh).  We also use Bouin's Fix, Biebrich Scarlet and Aniline Blue more than
once.  Is there a "set rule" when we should discard these solutions (i.e. after
5X use or 1 month)?

I would appreciate some feedback from the experts!


Peggy Sherwood
Lab Associate, Photopathology 
Wellman Center for Photomedicine (EDW 214)
Massachusetts General Hospital
55 Fruit Street
Boston, Massachusetts 02114-2696
617-724-4839 (voice mail)
617-726-6983 (lab)
617-726-1206 (fax)
msherwood <@t> partners.org

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