[Histonet] Dewaxing problem

Ceri Allen Ceri.Allen <@t> nottingham.ac.uk
Thu Aug 5 06:00:29 CDT 2010

Dear Histonetters,


I've recently started having a problem with tissue sections stubbornly
refusing to de-wax. I've been using  2 x 5 minutes in Histoclear to
dewax before re-hydrating and staining with normal H&E. Until about
three weeks ago this process worked fine. Leaving the sections in the
Histoclear for longer seems to have little effect and using Xylene
instead hasn't done much either - it's taking up to two days or more.
The type of tissue seems to be irrelevant, the sections are all cut at 5
microns, and I haven't changed any part of the processing. I've used
fresh alcohols and solvents, clean glassware - everything I can think


Can anyone give me any more ideas or possible causes? because i'm
stumped  L




Ceri Allen


Research Technician

School of Veterinary Medicine and Science

Sutton Bonnington Campus

University of Nottingham


Tel. 0115 95 16459

ceri.allen <@t> nottingham.ac.uk


This message has been checked for viruses but the contents of an attachment
may still contain software viruses which could damage your computer system:
you are advised to perform your own checks. Email communications with the
University of Nottingham may be monitored as permitted by UK legislation.

More information about the Histonet mailing list