[Histonet] Dewaxing problem

[Ceri Allen]

Dear Histonetters,

 

I've recently started having a problem with tissue sections stubbornly
refusing to de-wax. I've been using  2 x 5 minutes in Histoclear to
dewax before re-hydrating and staining with normal H&E. Until about
three weeks ago this process worked fine. Leaving the sections in the
Histoclear for longer seems to have little effect and using Xylene
instead hasn't done much either - it's taking up to two days or more.
The type of tissue seems to be irrelevant, the sections are all cut at 5
microns, and I haven't changed any part of the processing. I've used
fresh alcohols and solvents, clean glassware - everything I can think
of.

 

Can anyone give me any more ideas or possible causes? because i'm
stumped  L

 

Thanks

 

Ceri Allen

 

Research Technician

School of Veterinary Medicine and Science

Sutton Bonnington Campus

University of Nottingham

 

Tel. 0115 95 16459

ceri.allen <@t> nottingham.ac.uk

 

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