[Histonet] decalcification

[Sean McBride]

> Hi Dorothy,

I'm not sure what size bone specimens with which you are working, but I generally work with femurs & calvaria from rabbits, canine & other small rodents.  I fix my specimens longer in 10% NBF using an automated vacuum infiltration processor.  Following fixation, if decal is required, I will decal in buffered formic acid solution and use a calcium reagent set to titrate to the decalcification endpoint. If you would like any specifics, contact me offline.  

Best regards,


~Sean McBride


Senior Researcher
Bone Tissue Engineering Center
Carnegie Mellon Research Institute
Suite 4311
700 Technology Drive
Pittsburgh, PA 15219-3124

412-268-8275 (o)
412-915-1683 (m)
412-268-9807 (fax)
smcbride <@t> andrew.cmu.edu 

> 


 
> --- On Mon, 8/2/10, Webb, Dorothy L <Dorothy.L.Webb <@t> HealthPartners.Com> wrote:
> 
> 
> From: Webb, Dorothy L <Dorothy.L.Webb <@t> HealthPartners.Com>
> Subject: [Histonet] decalcification
> To: "'histonet <@t> lists.utsouthwestern.edu'" <histonet <@t> lists.utsouthwestern.edu>
> Date: Monday, August 2, 2010, 10:14 AM
> 
> 
> I would appreciate any feedback on what all are using in your decalcification process.  We get a lot of large bones in and the past 2-3 months have noticed a huge problem in our microtomy process with these samples.  We have been grossing the bones in and leaving the sample in the cassette in 10% formalin for 24 hours befoere placing in decal for up to 8 hours and still having the inner portion of the sample look underprocessed and crunchy!  Any suggestions would be appreciated!
> 
> Dorothy Webb, HT
> Regions Hospital Technical Supervisor
> 651-254-2962
> 
> 
> 
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