Rene J Buesa
rjbuesa <@t> yahoo.com
Mon Aug 2 10:55:21 CDT 2010
You cannot set a fixed time for decalcification. You have to leave the bone in the decalcifying solution until iy is soft and ready for processing.
--- On Mon, 8/2/10, Webb, Dorothy L <Dorothy.L.Webb <@t> HealthPartners.Com> wrote:
From: Webb, Dorothy L <Dorothy.L.Webb <@t> HealthPartners.Com>
Subject: [Histonet] decalcification
To: "'histonet <@t> lists.utsouthwestern.edu'" <histonet <@t> lists.utsouthwestern.edu>
Date: Monday, August 2, 2010, 10:14 AM
I would appreciate any feedback on what all are using in your decalcification process. We get a lot of large bones in and the past 2-3 months have noticed a huge problem in our microtomy process with these samples. We have been grossing the bones in and leaving the sample in the cassette in 10% formalin for 24 hours befoere placing in decal for up to 8 hours and still having the inner portion of the sample look underprocessed and crunchy! Any suggestions would be appreciated!
Dorothy Webb, HT
Regions Hospital Technical Supervisor
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