[Histonet] decalcification

Webb, Dorothy L Dorothy.L.Webb <@t> HealthPartners.Com
Mon Aug 2 09:14:35 CDT 2010

I would appreciate any feedback on what all are using in your decalcification process.  We get a lot of large bones in and the past 2-3 months have noticed a huge problem in our microtomy process with these samples.  We have been grossing the bones in and leaving the sample in the cassette in 10% formalin for 24 hours befoere placing in decal for up to 8 hours and still having the inner portion of the sample look underprocessed and crunchy!  Any suggestions would be appreciated!

Dorothy Webb, HT
Regions Hospital Technical Supervisor

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