[Histonet] Bone Marrow Clots - falling off slides

[Shirley A. Powell]

I cut the autopsies for the ME/FBI here and have a lot of bloody tissues I lovingly call "blood bricks".  I was using charged slides but these tissue would wash really badly.  I started using regular slides and StayOn from Surgipath and have had great success with keeping these bloody tissues on the slides.  I drain the water well, heat the slides up just to the point of melting the paraffin and dry them overnight in the slide dryer to make sure, but have also dried them for 45 minutes and they have stayed on.  
Shirley

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of histotech <@t> imagesbyhopper.com
Sent: Tuesday, April 27, 2010 7:33 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] Bone Marrow Clots - falling off slides

Mary,
Thanks for the suggestion.  We do check the alcohols with a hydrometer and
if not 100%, we will use that alcohol as the base for dilution alcohols.  We
check quality of the xylene by putting 10ml of water in a graduated cylinder
and then add 10ml of the recycled xylene to it and shake it vigorosly.
Good, clean xylene should have a perfect separation and no  "mixing" with
the water.

Lynette,
I am leaning towards the processing too, but which part??  The clot seems to
be more dry than moist, so it's not under dehydrated.  Fixation? It spends 3
hours in formalin on the tissue processor and whatever time it can be in
formalin prior to the processor.  Paraffin impregnation?  About the only
thing I could think of here was if the wax wasn't changed fast enough and/or
we got too much carry over from the xylene.  Could that cause this?

Thanks for the help!

Michelle



-----Original Message-----
From: Lynette Pavelich [mailto:lpaveli1 <@t> hurleymc.com] 
Sent: Tuesday, April 27, 2010 6:56 AM
To: histotech <@t> imagesbyhopper.com; histonet <@t> lists.utsouthwestern.edu; MARY
HODGES
Subject: RE: [Histonet] Bone Marrow Clots - falling off slides


When I start losing tissue on the slide, I have found that it is usually a
processing issue.  Is the clot too thick?  Try cutting them in half before
processing. 

Hope this helped,
Lynette

Lynette Pavelich, HT(ASCP)
Histology Supervisor
MSH Competency Coordinator
Hurley Medical Center
One Hurley Plaza
Flint, MI  48503
email: Lpaveli1 <@t> hurleymc.com
ph:  810-257-9948
Lab:  810-257-9138
fax:  810-762-7082


>>> MARY T HODGES <hodges420 <@t> msn.com> 4/27/2010 12:18 AM >>>

recycled anything is not back to it original state always use a hydo meter
and add new to back product of a better quality you will find this also with
alcohols
 
> From: histotech <@t> imagesbyhopper.com
> To: histonet <@t> lists.utsouthwestern.edu 
> Date: Mon, 26 Apr 2010 23:26:43 -0400
> Subject: [Histonet] Bone Marrow Clots - falling off slides
> 
> Hi Histonetters!
> 
> We have started to have an issue with our bone marrow clots falling
off the
> slides. We are using plus slides, making sure they drain well (just
like
> our other slides), but when we stain them routinely, we are getting a
fair
> amount of tissue coming off the slides.
> 
> It has been suggested that it's related to our using recycled xylene.
Does
> anyone have any experience with recycled xylene and this type of
tissue
> fall-off?
> 
> Another suggestion was that the tissues sat in xylene too long. I
don't see
> how that could happen, under routine conditions, but is that a
possibility
> for causing this?
> 
> All thoughts will be appreciated!
> 
> Michelle
> 
> 
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> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet 
 		 	   		  
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