[Histonet] Bone Marrow Clots - falling off slides

Tue Apr 27 08:20:29 CDT 2010

Thanks for all the input.  I will check the collection method.  B-plus  
is the fixative we normally use but I am not convinced that this clot  
ended up in it!  Other tissues are not being dramatically affected and  
not even all the bone marrows!  We do soak the blocks too.

This is very frustrating.  :o(

Michelle

On Apr 27, 2010, at 7:59 AM, "Lynette Pavelich"  
<lpaveli1 <@t> hurleymc.com> wrote:

> In my experience, it can be all of the above that can cause it.  By  
> the
> tissue not getting enough penetration by any of the solutions will  
> alter
> the outcome.  That is why I asked about the thickness (remember...only
> as thick as a nickel).  Blood is also so dense, so it would really  
> apply
> here.  Try that.  Do 1 change at a time.  I've been in your shoes,  
> it is
> very frustrating.  Then if the thickness is good, work on something
> else.....starting with fixation times.
>
>
>>>> <histotech <@t> imagesbyhopper.com> 4/27/2010 7:32 AM >>>
> Mary,
> Thanks for the suggestion.  We do check the alcohols with a hydrometer
> and
> if not 100%, we will use that alcohol as the base for dilution
> alcohols.  We
> check quality of the xylene by putting 10ml of water in a graduated
> cylinder
> and then add 10ml of the recycled xylene to it and shake it vigorosly.
> Good, clean xylene should have a perfect separation and no  "mixing"
> with
> the water.
>
> Lynette,
> I am leaning towards the processing too, but which part??  The clot
> seems to
> be more dry than moist, so it's not under dehydrated.  Fixation? It
> spends 3
> hours in formalin on the tissue processor and whatever time it can be
> in
> formalin prior to the processor.  Paraffin impregnation?  About the
> only
> thing I could think of here was if the wax wasn't changed fast enough
> and/or
> we got too much carry over from the xylene.  Could that cause this?
>
> Thanks for the help!
>
> Michelle
>
>
>
> -----Original Message-----
> From: Lynette Pavelich [mailto:lpaveli1 <@t> hurleymc.com]
> Sent: Tuesday, April 27, 2010 6:56 AM
> To: histotech <@t> imagesbyhopper.com; histonet <@t> lists.utsouthwestern.edu;
> MARY
> HODGES
> Subject: RE: [Histonet] Bone Marrow Clots - falling off slides
>
>
> When I start losing tissue on the slide, I have found that it is
> usually a
> processing issue.  Is the clot too thick?  Try cutting them in half
> before
> processing.
>
> Hope this helped,
> Lynette
>
> Lynette Pavelich, HT(ASCP)
> Histology Supervisor
> MSH Competency Coordinator
> Hurley Medical Center
> One Hurley Plaza
> Flint, MI  48503
> email: Lpaveli1 <@t> hurleymc.com
> ph:  810-257-9948
> Lab:  810-257-9138
> fax:  810-762-7082
>
>
>>>> MARY T HODGES <hodges420 <@t> msn.com> 4/27/2010 12:18 AM >>>
>
> recycled anything is not back to it original state always use a hydo
> meter
> and add new to back product of a better quality you will find this  
> also
> with
> alcohols
>
>> From: histotech <@t> imagesbyhopper.com
>> To: histonet <@t> lists.utsouthwestern.edu
>> Date: Mon, 26 Apr 2010 23:26:43 -0400
>> Subject: [Histonet] Bone Marrow Clots - falling off slides
>>
>> Hi Histonetters!
>>
>> We have started to have an issue with our bone marrow clots falling
> off the
>> slides. We are using plus slides, making sure they drain well (just
> like
>> our other slides), but when we stain them routinely, we are getting
> a
> fair
>> amount of tissue coming off the slides.
>>
>> It has been suggested that it's related to our using recycled
> xylene.
> Does
>> anyone have any experience with recycled xylene and this type of
> tissue
>> fall-off?
>>
>> Another suggestion was that the tissues sat in xylene too long. I
> don't see
>> how that could happen, under routine conditions, but is that a
> possibility
>> for causing this?
>>
>> All thoughts will be appreciated!
>>
>> Michelle
>>
>>
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