[Histonet] Bone Marrow Clots - falling off slides

Tue Apr 27 07:59:07 CDT 2010

In my experience, it can be all of the above that can cause it.  By the
tissue not getting enough penetration by any of the solutions will alter
the outcome.  That is why I asked about the thickness (remember...only
as thick as a nickel).  Blood is also so dense, so it would really apply
here.  Try that.  Do 1 change at a time.  I've been in your shoes, it is
very frustrating.  Then if the thickness is good, work on something
else.....starting with fixation times.

>>> <histotech <@t> imagesbyhopper.com> 4/27/2010 7:32 AM >>>
Mary,
Thanks for the suggestion.  We do check the alcohols with a hydrometer
and
if not 100%, we will use that alcohol as the base for dilution
alcohols.  We
check quality of the xylene by putting 10ml of water in a graduated
cylinder
and then add 10ml of the recycled xylene to it and shake it vigorosly.
Good, clean xylene should have a perfect separation and no  "mixing"
with
the water.

Lynette,
I am leaning towards the processing too, but which part??  The clot
seems to
be more dry than moist, so it's not under dehydrated.  Fixation? It
spends 3
hours in formalin on the tissue processor and whatever time it can be
in
formalin prior to the processor.  Paraffin impregnation?  About the
only
thing I could think of here was if the wax wasn't changed fast enough
and/or
we got too much carry over from the xylene.  Could that cause this?

Thanks for the help!

Michelle

-----Original Message-----
From: Lynette Pavelich [mailto:lpaveli1 <@t> hurleymc.com] 
Sent: Tuesday, April 27, 2010 6:56 AM
To: histotech <@t> imagesbyhopper.com; histonet <@t> lists.utsouthwestern.edu;
MARY
HODGES
Subject: RE: [Histonet] Bone Marrow Clots - falling off slides

When I start losing tissue on the slide, I have found that it is
usually a
processing issue.  Is the clot too thick?  Try cutting them in half
before
processing. 

Hope this helped,
Lynette

Lynette Pavelich, HT(ASCP)
Histology Supervisor
MSH Competency Coordinator
Hurley Medical Center
One Hurley Plaza
Flint, MI  48503
email: Lpaveli1 <@t> hurleymc.com 
ph:  810-257-9948
Lab:  810-257-9138
fax:  810-762-7082

>>> MARY T HODGES <hodges420 <@t> msn.com> 4/27/2010 12:18 AM >>>

recycled anything is not back to it original state always use a hydo
meter
and add new to back product of a better quality you will find this also
with
alcohols

> From: histotech <@t> imagesbyhopper.com 
> To: histonet <@t> lists.utsouthwestern.edu 
> Date: Mon, 26 Apr 2010 23:26:43 -0400
> Subject: [Histonet] Bone Marrow Clots - falling off slides
> 
> Hi Histonetters!
> 
> We have started to have an issue with our bone marrow clots falling
off the
> slides. We are using plus slides, making sure they drain well (just
like
> our other slides), but when we stain them routinely, we are getting
a
fair
> amount of tissue coming off the slides.
> 
> It has been suggested that it's related to our using recycled
xylene.
Does
> anyone have any experience with recycled xylene and this type of
tissue
> fall-off?
> 
> Another suggestion was that the tissues sat in xylene too long. I
don't see
> how that could happen, under routine conditions, but is that a
possibility
> for causing this?
> 
> All thoughts will be appreciated!
> 
> Michelle
> 
> 
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu 
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet 

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