[Histonet] Bone Marrow Clots - falling off slides

[MARY T HODGES]

recycled anything is not back to it original state always use a hydo meter and add new to back product of a better quality you will find this also with alcohols
 
> From: histotech <@t> imagesbyhopper.com
> To: histonet <@t> lists.utsouthwestern.edu
> Date: Mon, 26 Apr 2010 23:26:43 -0400
> Subject: [Histonet] Bone Marrow Clots - falling off slides
> 
> Hi Histonetters!
> 
> We have started to have an issue with our bone marrow clots falling off the
> slides. We are using plus slides, making sure they drain well (just like
> our other slides), but when we stain them routinely, we are getting a fair
> amount of tissue coming off the slides.
> 
> It has been suggested that it's related to our using recycled xylene. Does
> anyone have any experience with recycled xylene and this type of tissue
> fall-off?
> 
> Another suggestion was that the tissues sat in xylene too long. I don't see
> how that could happen, under routine conditions, but is that a possibility
> for causing this?
> 
> All thoughts will be appreciated!
> 
> Michelle
> 
> 
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