[Histonet] Bone Marrow Clots - falling off slides

Lynette Pavelich lpaveli1 <@t> hurleymc.com
Tue Apr 27 05:55:32 CDT 2010


When I start losing tissue on the slide, I have found that it is usually
a processing issue.  Is the clot too thick?  Try cutting them in half
before processing. 

Hope this helped,
Lynette

Lynette Pavelich, HT(ASCP)
Histology Supervisor
MSH Competency Coordinator
Hurley Medical Center
One Hurley Plaza
Flint, MI  48503
email: Lpaveli1 <@t> hurleymc.com
ph:  810-257-9948
Lab:  810-257-9138
fax:  810-762-7082


>>> MARY T HODGES <hodges420 <@t> msn.com> 4/27/2010 12:18 AM >>>

recycled anything is not back to it original state always use a hydo
meter and add new to back product of a better quality you will find this
also with alcohols
 
> From: histotech <@t> imagesbyhopper.com 
> To: histonet <@t> lists.utsouthwestern.edu 
> Date: Mon, 26 Apr 2010 23:26:43 -0400
> Subject: [Histonet] Bone Marrow Clots - falling off slides
> 
> Hi Histonetters!
> 
> We have started to have an issue with our bone marrow clots falling
off the
> slides. We are using plus slides, making sure they drain well (just
like
> our other slides), but when we stain them routinely, we are getting a
fair
> amount of tissue coming off the slides.
> 
> It has been suggested that it's related to our using recycled xylene.
Does
> anyone have any experience with recycled xylene and this type of
tissue
> fall-off?
> 
> Another suggestion was that the tissues sat in xylene too long. I
don't see
> how that could happen, under routine conditions, but is that a
possibility
> for causing this?
> 
> All thoughts will be appreciated!
> 
> Michelle
> 
> 
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