[Histonet] Bone marrow trephine decalcification

[McMahon, Loralee A]

They have commercial rapid decals that are formic acid based that do not affect the immunohistochemistry.  But it does affect some of the enzyme histochemistry.  


Loralee McMahon, HTL (ASCP)
Immunohistochemistry Supervisor
Strong Memorial Hospital
Department of Surgical Pathology
(585) 275-7210
________________________________________
From: histonet-bounces <@t> lists.utsouthwestern.edu [histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Marinez [mbbarra <@t> uol.com.br]
Sent: Wednesday, April 21, 2010 4:14 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Bone marrow trephine decalcification

I'm in a bit of trouble. I work in the south of Brazil (Porto Alegre) for some years and now in my hospital a lot of bone marrow biopsies are being performed (leukemia and lymphomas). With decalcification with strong acids (nitric) I'm getting very poor results with the immunohistochemistry. I'm trying to use EDTA but we really are not getting speed or good results (we  controled de pH but still precipitates). Could some one be kind enough to send me some formula (simple one please) that will work in reasonable time (24 or 48 hours) and perform well with immunohistochemistry? I would deeply grateful.



M. Barra
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