[Histonet] Bone marrow trephine decalcification

[Malika Benatti]

Goodings & Stewarts also known as Formic/Formaldehyde fluid (10%  
formic acid, 5% formalin) for 6 hours  should allow decal of fix BMT  
trephine Bx.




" ... Smile it confuses people ..."

On 21 Apr 2010, at 21:14, "Marinez" <mbbarra <@t> uol.com.br> wrote:

> I'm in a bit of trouble. I work in the south of Brazil (Porto  
> Alegre) for some years and now in my hospital a lot of bone marrow  
> biopsies are being performed (leukemia and lymphomas). With  
> decalcification with strong acids (nitric) I'm getting very poor  
> results with the immunohistochemistry. I'm trying to use EDTA but we  
> really are not getting speed or good results (we  controled de pH  
> but still precipitates). Could some one be kind enough to send me  
> some formula (simple one please) that will work in reasonable time  
> (24 or 48 hours) and perform well with immunohistochemistry? I would  
> deeply grateful.
>
>
>
> M. Barra
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