[Histonet] Bone marrow trephine decalcification
Malika Benatti
malbenatti <@t> googlemail.com
Wed Apr 21 15:33:48 CDT 2010
Goodings & Stewarts also known as Formic/Formaldehyde fluid (10%
formic acid, 5% formalin) for 6 hours should allow decal of fix BMT
trephine Bx.
" ... Smile it confuses people ..."
On 21 Apr 2010, at 21:14, "Marinez" <mbbarra <@t> uol.com.br> wrote:
> I'm in a bit of trouble. I work in the south of Brazil (Porto
> Alegre) for some years and now in my hospital a lot of bone marrow
> biopsies are being performed (leukemia and lymphomas). With
> decalcification with strong acids (nitric) I'm getting very poor
> results with the immunohistochemistry. I'm trying to use EDTA but we
> really are not getting speed or good results (we controled de pH
> but still precipitates). Could some one be kind enough to send me
> some formula (simple one please) that will work in reasonable time
> (24 or 48 hours) and perform well with immunohistochemistry? I would
> deeply grateful.
>
>
>
> M. Barra
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