[Histonet] RE: CD31 in pigs

[Merced M Leiker]

Me three, please.  :-)

I just discovered we have this same antibody in our lab but my boss was 
unsuccessful with it (he had tried 95oC for 45 min, pH 6.0).

Thanks!

Merced

--On Tuesday, April 13, 2010 2:24 PM -0500 shive003 <@t> umn.edu wrote:

> Me, too, please.
>
> Jan Shivers
> UMN Vet Diag Lab
>
> On Apr 12 2010, Liz Wilson wrote:
>
>> Hi Patsy,
>> I would love to try the method!  Thanks -Liz
>>
>> Liz Wilson
>> Bethyl Labs
>>
>>
>> ________________________________________________________________________
>> ____ Message: 7
>> Date: Sat, 10 Apr 2010 10:33:02 -0600
>> From: "Patsy Ruegg" <pruegg <@t> ihctech.net>
>> Subject: RE: SPAM-LOW:  [Histonet] Re: Cd31 in pigs
>> To: "'Liz Wilson'" <lwilson <@t> bethyl.com>,
>>	<histonet <@t> lists.utsouthwestern.edu>
>> Message-ID: <59ADBA33D53B44778565F88CBD7FA13A <@t> prueggihctechlt>
>> Content-Type: text/plain;	charset="us-ascii"
>>
>> A few years ago I spent a lot of effort on using Serotec's mouse
>> anti-porcine CD31 for ffpe porcine tissue and I did get some positive
>> results with unusual pretreatment methods, as I recall I had to use a
>> waterbath for hier set at 70dc for 3 hours in hph buffer.  I will look up
>> the method at work on Monday and send it to you if you contact me again
>> to remind me.
>>
>> Patsy
>>
>> Patsy Ruegg, HT(ASCP)QIHC
>> IHCtech
>> 12635 Montview Blvd. Ste.215
>> Aurora, CO 80045
>> 720-859-4060
>> fax 720-859-4110
>> www.ihctech.net
>> www.ihcrg.org
>>
>>
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>
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Merced M Leiker
Research Technician III
Cardiovascular Medicine
348 Biomedical Research Building
State University of New York at Buffalo
3435 Main St, Buffalo, NY 14214  USA
leiker <@t> buffalo.edu
716-829-6118 (Ph)
716-829-2665 (Fx)

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