[Histonet] RE: CD31 in pigs

Tue Apr 13 14:24:53 CDT 2010

Me, too, please.

Jan Shivers
UMN Vet Diag Lab

On Apr 12 2010, Liz Wilson wrote:

>Hi Patsy,
>I would love to try the method!  Thanks -Liz
>
>Liz Wilson
>Bethyl Labs
>
>  
> ____________________________________________________________________________
>Message: 7
>Date: Sat, 10 Apr 2010 10:33:02 -0600
>From: "Patsy Ruegg" <pruegg <@t> ihctech.net>
>Subject: RE: SPAM-LOW:  [Histonet] Re: Cd31 in pigs
>To: "'Liz Wilson'" <lwilson <@t> bethyl.com>,
>	<histonet <@t> lists.utsouthwestern.edu>
>Message-ID: <59ADBA33D53B44778565F88CBD7FA13A <@t> prueggihctechlt>
>Content-Type: text/plain;	charset="us-ascii"
>
>A few years ago I spent a lot of effort on using Serotec's mouse
>anti-porcine CD31 for ffpe porcine tissue and I did get some positive
>results with unusual pretreatment methods, as I recall I had to use a
>waterbath for hier set at 70dc for 3 hours in hph buffer.  I will look up
>the method at work on Monday and send it to you if you contact me again to
>remind me.
>
>Patsy
>
>Patsy Ruegg, HT(ASCP)QIHC
>IHCtech
>12635 Montview Blvd. Ste.215
>Aurora, CO 80045
>720-859-4060
>fax 720-859-4110
>www.ihctech.net
>www.ihcrg.org
>
>
>_______________________________________________
>Histonet mailing list
>Histonet <@t> lists.utsouthwestern.edu
>http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>