[Histonet] RE: CD31 in pigs

[iskaliora <@t> bioacademy.gr]

please add me to the growing list as well.
thanks,
IS.

-----------------------------------------------------
Irini Skaliora, PhD
Research Assistant Professor
Developmental Biology Division
Biomedical Research Foundation of the Academy of Athens (BRFAA)
Soranou Efessiou 4
Athens 11527
tel. +30-210-6597203 (office)
tel. +30-210-6597482 (lab)
fax. +30-210-6597545
email: iskaliora <@t> bioacademy.gr

> Me three, please.  :-)
>
> I just discovered we have this same antibody in our lab but my boss was
> unsuccessful with it (he had tried 95oC for 45 min, pH 6.0).
>
> Thanks!
>
> Merced
>
> --On Tuesday, April 13, 2010 2:24 PM -0500 shive003 <@t> umn.edu wrote:
>
>> Me, too, please.
>>
>> Jan Shivers
>> UMN Vet Diag Lab
>>
>> On Apr 12 2010, Liz Wilson wrote:
>>
>>> Hi Patsy,
>>> I would love to try the method!  Thanks -Liz
>>>
>>> Liz Wilson
>>> Bethyl Labs
>>>
>>>
>>> ________________________________________________________________________
>>> ____ Message: 7
>>> Date: Sat, 10 Apr 2010 10:33:02 -0600
>>> From: "Patsy Ruegg" <pruegg <@t> ihctech.net>
>>> Subject: RE: SPAM-LOW:  [Histonet] Re: Cd31 in pigs
>>> To: "'Liz Wilson'" <lwilson <@t> bethyl.com>,
>>>	<histonet <@t> lists.utsouthwestern.edu>
>>> Message-ID: <59ADBA33D53B44778565F88CBD7FA13A <@t> prueggihctechlt>
>>> Content-Type: text/plain;	charset="us-ascii"
>>>
>>> A few years ago I spent a lot of effort on using Serotec's mouse
>>> anti-porcine CD31 for ffpe porcine tissue and I did get some positive
>>> results with unusual pretreatment methods, as I recall I had to use a
>>> waterbath for hier set at 70dc for 3 hours in hph buffer.  I will look
>>> up
>>> the method at work on Monday and send it to you if you contact me again
>>> to remind me.
>>>
>>> Patsy
>>>
>>> Patsy Ruegg, HT(ASCP)QIHC
>>> IHCtech
>>> 12635 Montview Blvd. Ste.215
>>> Aurora, CO 80045
>>> 720-859-4060
>>> fax 720-859-4110
>>> www.ihctech.net
>>> www.ihcrg.org
>>>
>>>
>>> _______________________________________________
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>>> Histonet <@t> lists.utsouthwestern.edu
>>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>>>
>>
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>
>
>
> Merced M Leiker
> Research Technician III
> Cardiovascular Medicine
> 348 Biomedical Research Building
> State University of New York at Buffalo
> 3435 Main St, Buffalo, NY 14214  USA
> leiker <@t> buffalo.edu
> 716-829-6118 (Ph)
> 716-829-2665 (Fx)
>
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