[Histonet] RE: CD31 in pigs

Mon Apr 12 11:54:13 CDT 2010

 Ditto!!

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Joel Israel
Sent: Monday, April 12, 2010 9:22 AM
To: Liz Wilson; histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] RE: CD31 in pigs

Please count me in too!  I would love a procedure that works and has been tested.  Thanks- Joel 

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Liz Wilson
Sent: Monday, April 12, 2010 10:08 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] RE: CD31 in pigs

Hi Patsy,
I would love to try the method!  Thanks -Liz

Liz Wilson
Bethyl Labs

________________________________________________________________________
____
Message: 7
Date: Sat, 10 Apr 2010 10:33:02 -0600
From: "Patsy Ruegg" <pruegg <@t> ihctech.net>
Subject: RE: SPAM-LOW:  [Histonet] Re: Cd31 in pigs
To: "'Liz Wilson'" <lwilson <@t> bethyl.com>,
	<histonet <@t> lists.utsouthwestern.edu>
Message-ID: <59ADBA33D53B44778565F88CBD7FA13A <@t> prueggihctechlt>
Content-Type: text/plain;	charset="us-ascii"

A few years ago I spent a lot of effort on using Serotec's mouse anti-porcine CD31 for ffpe porcine tissue and I did get some positive results with unusual pretreatment methods, as I recall I had to use a waterbath for hier set at 70dc for 3 hours in hph buffer.  I will look up the method at work on Monday and send it to you if you contact me again to remind me.

Patsy

Patsy Ruegg, HT(ASCP)QIHC
IHCtech
12635 Montview Blvd. Ste.215
Aurora, CO 80045
720-859-4060
fax 720-859-4110
www.ihctech.net
www.ihcrg.org

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