[Histonet] RE: CD31 in pigs

[Liz Wilson]

Hi Patsy,
I would love to try the method!  Thanks -Liz

Liz Wilson
Bethyl Labs

____________________________________________________________________________
Message: 7
Date: Sat, 10 Apr 2010 10:33:02 -0600
From: "Patsy Ruegg" <pruegg <@t> ihctech.net>
Subject: RE: SPAM-LOW:  [Histonet] Re: Cd31 in pigs
To: "'Liz Wilson'" <lwilson <@t> bethyl.com>,
	<histonet <@t> lists.utsouthwestern.edu>
Message-ID: <59ADBA33D53B44778565F88CBD7FA13A <@t> prueggihctechlt>
Content-Type: text/plain;	charset="us-ascii"

A few years ago I spent a lot of effort on using Serotec's mouse
anti-porcine CD31 for ffpe porcine tissue and I did get some positive
results with unusual pretreatment methods, as I recall I had to use a
waterbath for hier set at 70dc for 3 hours in hph buffer.  I will look up
the method at work on Monday and send it to you if you contact me again to
remind me.

Patsy

Patsy Ruegg, HT(ASCP)QIHC
IHCtech
12635 Montview Blvd. Ste.215
Aurora, CO 80045
720-859-4060
fax 720-859-4110
www.ihctech.net
www.ihcrg.org