SPAM-LOW: Re: [Histonet] Cd31 in pigs

[Patsy Ruegg]

Yea you can use F8 and SMA for vessel lining cells but those will not pick
up all the developing endothelial cells like cd31 or cd34.

There is a new rat anti mouse CD31 from Japan, I wonder it if works on pig,
I will try it and see, it works great on mouse tissue ffpe.

Regards,

Patsy

Patsy Ruegg, HT(ASCP)QIHC
IHCtech
12635 Montview Blvd. Ste.215
Aurora, CO 80045
720-859-4060
fax 720-859-4110
www.ihctech.net 
www.ihcrg.org


-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Jan Shivers
Sent: Friday, April 09, 2010 10:24 AM
To: Jean-Martin Lapointe; histonet <@t> lists.utsouthwestern.edu
Subject: SPAM-LOW: Re: [Histonet] Cd31 in pigs

Von Willebrand Factor (Factor VIII) is also what I use to stain pig 
endothelium.

Jan Shivers
Senior Scientist
Pathology Teaching Program
Histology/IHC/EM Section Head
University of Minnesota
Veterinary Diagnostic Laboratory
1333 Gortner Ave.
St. Paul, MN  55108
612-624-7297
shive003 <@t> umn.edu

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----- Original Message ----- 
From: "Jean-Martin Lapointe" <jm.lapointe <@t> accellab.com>
To: <histonet <@t> lists.utsouthwestern.edu>
Sent: Friday, April 09, 2010 10:59 AM
Subject: [Histonet] Cd31 in pigs


Hi Joel,
we have tried a few antibodies for CD31 in FFPE tissues in pigs and have had

no luck. We currently use an antibody to vWF to stain pig endothelium. If 
someone has a working technique for CD31 in swine, I'd be happy to hear 
about it.

Jean-Martin
__________________________________
Jean-Martin Lapointe, DMV, MS, dACVP
AccelLAB Inc
Québec, Canada  J7H 1N8
jm.lapointe <@t> accellab.com



Date: Fri, 9 Apr 2010 09:40:00 -0400
From: "Joel Israel" <JoelI <@t> mcclainlab.com>


Does anyone have a procedure that WORKS for cd31 in pigs?  I can't seem
to get it to work no matter what I do.  Any help will be greatly
appreciated.  Thanks.- joel



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