SPAM-LOW: Re: [Histonet] Cd31 in pigs

[Liz Chlipala]

Patsy

How do you order that rat anti-mouse CD31.

Thanks

Liz

Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Manager
Premier Laboratory, LLC
PO Box 18592
Boulder, Colorado 80308
office (303) 682-3949 
fax (303) 682-9060
www.premierlab.com
 
 
Ship to Address:
1567 Skyway Drive, Unit E
Longmont, Colorado 80504

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Patsy Ruegg
Sent: Saturday, April 10, 2010 10:40 AM
To: 'Jan Shivers'; 'Jean-Martin Lapointe'; histonet <@t> lists.utsouthwestern.edu
Subject: RE: SPAM-LOW: Re: [Histonet] Cd31 in pigs

Yea you can use F8 and SMA for vessel lining cells but those will not pick
up all the developing endothelial cells like cd31 or cd34.

There is a new rat anti mouse CD31 from Japan, I wonder it if works on pig,
I will try it and see, it works great on mouse tissue ffpe.

Regards,

Patsy

Patsy Ruegg, HT(ASCP)QIHC
IHCtech
12635 Montview Blvd. Ste.215
Aurora, CO 80045
720-859-4060
fax 720-859-4110
www.ihctech.net 
www.ihcrg.org


-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Jan Shivers
Sent: Friday, April 09, 2010 10:24 AM
To: Jean-Martin Lapointe; histonet <@t> lists.utsouthwestern.edu
Subject: SPAM-LOW: Re: [Histonet] Cd31 in pigs

Von Willebrand Factor (Factor VIII) is also what I use to stain pig 
endothelium.

Jan Shivers
Senior Scientist
Pathology Teaching Program
Histology/IHC/EM Section Head
University of Minnesota
Veterinary Diagnostic Laboratory
1333 Gortner Ave.
St. Paul, MN  55108
612-624-7297
shive003 <@t> umn.edu

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----- Original Message ----- 
From: "Jean-Martin Lapointe" <jm.lapointe <@t> accellab.com>
To: <histonet <@t> lists.utsouthwestern.edu>
Sent: Friday, April 09, 2010 10:59 AM
Subject: [Histonet] Cd31 in pigs


Hi Joel,
we have tried a few antibodies for CD31 in FFPE tissues in pigs and have had

no luck. We currently use an antibody to vWF to stain pig endothelium. If 
someone has a working technique for CD31 in swine, I'd be happy to hear 
about it.

Jean-Martin
__________________________________
Jean-Martin Lapointe, DMV, MS, dACVP
AccelLAB Inc
Québec, Canada  J7H 1N8
jm.lapointe <@t> accellab.com



Date: Fri, 9 Apr 2010 09:40:00 -0400
From: "Joel Israel" <JoelI <@t> mcclainlab.com>


Does anyone have a procedure that WORKS for cd31 in pigs?  I can't seem
to get it to work no matter what I do.  Any help will be greatly
appreciated.  Thanks.- joel



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