[Histonet] staining of plastic sections

[kbowden]

I use McNeal as a substitute for H&E.  I use it on MMA embedded bone.  
It is to intense (for us) to be used on JB-4.  I think you might need to 
try a few different stains to select the best.

*/ /*

*/-- You are what you do - not what you say --/*

*/Karen Bowden
Staff Research Associate II
University of CA, San Diego
Department of Orthopedics
3525 John Hopkins Ct. 0863
San Diego, CA 92121-08630
858-822-1251 voice
858-534-5304 fax


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Walters, Katherine S wrote:
> Perhaps you could try the Paragon stain.  It looks more like an H&E.
>
> Kathy Walters
>
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu
> [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Lesley
> Bechtold
> Sent: Wednesday, September 30, 2009 10:12 AM
> To: histonet <@t> lists.utsouthwestern.edu
> Cc: Pete Finger
> Subject: [Histonet] staining of plastic sections 
>
> Hi Histonetters,
>
> If anyone has a good protocol for staining plastic sections, could they
> share it?  We have samples embedded in an epoxy resin for TEM but they
> love the semi-thick sections on the glass slide and asked if we could do
> something other than a toluidine blue O, the standard stain for plastic.
> We have tried H&E and PAS with no success.  If there some way to treat
> the sections in order to get them to stain?  Is there another stain we
> could try?
>
> Thank you!
>
> Lesley
>
>
> Lesley S. Bechtold
> Senior Manager, Histopathology Sciences
> The Jackson Laboratory
> 600 Main St.
> Bar Harbor, ME 04609
> 207-288-6322 (phone)
> 207-288-6325 (fax)
>
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