[Histonet] staining of plastic sections

Geoff McAuliffe mcauliff <@t> umdnj.edu
Wed Sep 30 10:41:16 CDT 2009

Hi Lesley:

There is a fairly large literature on this subject. I my opinion, most 
of the fancier stains are not worth the effort and the results are 
disappointing. In a separate mailing, I will send you some references.


Lesley Bechtold wrote:
> Hi Histonetters,
> If anyone has a good protocol for staining plastic sections, could they share it?  We have samples embedded in an epoxy resin for TEM but they love the semi-thick sections on the glass slide and asked if we could do something other than a toluidine blue O, the standard stain for plastic.  We have tried H&E and PAS with no success.  If there some way to treat the sections in order to get them to stain?  Is there another stain we could try?
> Thank you!
> Lesley
> Lesley S. Bechtold
> Senior Manager, Histopathology Sciences
> The Jackson Laboratory
> 600 Main St.
> Bar Harbor, ME 04609
> 207-288-6322 (phone)
> 207-288-6325 (fax)
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Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583 
mcauliff <@t> umdnj.edu

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