[Histonet] staining of plastic sections

Lesley Bechtold Lesley.Bechtold <@t> jax.org
Wed Sep 30 10:11:42 CDT 2009

Hi Histonetters,

If anyone has a good protocol for staining plastic sections, could they share it?  We have samples embedded in an epoxy resin for TEM but they love the semi-thick sections on the glass slide and asked if we could do something other than a toluidine blue O, the standard stain for plastic.  We have tried H&E and PAS with no success.  If there some way to treat the sections in order to get them to stain?  Is there another stain we could try?

Thank you!


Lesley S. Bechtold
Senior Manager, Histopathology Sciences
The Jackson Laboratory
600 Main St.
Bar Harbor, ME 04609
207-288-6322 (phone)
207-288-6325 (fax)

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