[Histonet] COX staining in frosen muscles

[Florence Leomiti]

No Problem Delores

Send me your fax number and I will send over our protocol.

Florence Leomiti    HT (ASCP)
OHSU Neuromuscular Lab Tech.
Phone 503-494-6781
Fax 503-494-6787
Pager 16822

-----Original Message-----
From: Diaz, Dolores [mailto:dolores.diaz <@t> ttuhsc.edu] 
Sent: Monday, September 28, 2009 12:41 PM
To: Florence Leomiti
Subject: RE: [Histonet] COX staining in frosen muscles

Hello, Florence

I work in a research facility and we are beginning to stain frozen rat muscle. I will attempt to stain for COX and would also love to have a copy of your protocol if it isn't asking too much.

Thank you,
Dolores Diaz HT (ASCP)
TTUHSC

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Florence Leomiti
Sent: Monday, September 28, 2009 12:51 PM
To: Galina Deyneko; histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] COX staining in frosen muscles

Hi Galina

I work in the neuromuscular lab and perform this stain on a weekly basis I have a protocol I can share with you. Just contact me and I can send it on.


Florence Leomiti    HT (ASCP)
OHSU Neuromuscular Lab Tech.
Phone 503-494-6781
Fax 503-494-6787
Pager 16822


-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Galina Deyneko
Sent: Monday, September 28, 2009 11:42 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] COX staining in frosen muscles



Dear Colleagues.
Please help. I have been asking to perform staining for  COX (which is cytochrome oxidase I believe) activity on mouse fresh frozen leg muscles. I am not very  familiar with enzyme histochemistry. I found one method (Seligman,1968) in J.Bancroft , 5 edition book, and the other in J. Kiernan "Histochemical Methods" book, but the methods are described for the fixed brain. I  am absolutely not sure that I have found the required methods. 
Could you share with me your experience, detailed protocol or the sources where i can find the reliable protocol siutable for muscle histochemistry.Thank you in advance.
Galina Deyneko
Novartis Cambridge, MA


      
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