[Histonet] Guniea pig anti-Doublecortin & Goat-Guinea pig
secondaryantibody on rat tissue
tifei <@t> foxmail.com
Mon Sep 28 15:16:22 CDT 2009
Subject: [Histonet] Guniea pig anti-Doublecortin & Goat-Guinea pig secondaryantibody on rat tissue
it seems when I use guniea pig-anti-doublecortin (DCX) with secondary antibody from invitrogen goat-anti guinea pig Alexa 488 or 568. I always get staining on blood vessel-like structures.
Anyone has such experiences before? Do goat-anti-guinea pig IgG will cross react with rat IgG ?
Or it is due to primary antibody problem - which is unlikely because the antibody has been used without a problem in other labs.
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