[Histonet] Re: Permount question

[Merced M Leiker]

I do recall having to do that in the past; if the Permount bottle had been 
left open in the hood and became too viscous we would add just enough 
xylene to get it flowing again. Didn't happen often, if you're careful to 
keep the bottle closed when not in use.

Regards,
Merced

--On Monday, September 14, 2009 9:15 AM +1000 Tony Henwood 
<AnthonyH <@t> chw.edu.au> wrote:

> It is possible that the mountant was too thick and so xylene was added
> to thin it?
>
> It would save the lab money in mountant costs but there is a possibility
> that with less mountant on the slide you may have shrinkage of the
> mountant causing eventual cracking of the mountant and possible
> detachment of the coverslip.
>
> I would review a sample of slides one and two years old and record the
> quality (another audit!)
>
> Regards
>
> Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC)
> Laboratory Manager & Senior Scientist
> Tel: 612 9845 3306
> Fax: 612 9845 3318
> the children's hospital at westmead
> Cnr Hawkesbury Road and Hainsworth Street, Westmead
> Locked Bag 4001, Westmead NSW 2145, AUSTRALIA
>
>
>
>
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu
> [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Merced M
> Leiker
> Sent: Saturday, 12 September 2009 12:30 AM
> To: Leslie Chen; histonet <@t> lists.utsouthwestern.edu
> Subject: Re: [Histonet] Re: Permount question
>
>
> Just so a stereotype doesn't get started here...I was 1st taught in the
> (straight) use of Permount by such an Asian technician (who couldn't
> speak
> English, but was such a wonderful teacher I learned more from watching
> her
> than anything!)  :-)
>
>
> --On Thursday, September 10, 2009 1:52 PM -0700 Leslie Chen
> <lchen <@t> mednet.ucla.edu> wrote:
>
>> Thanks for all the replies, I guess these weird Asian technicians who
>> are highly looked up to taught me something wrong and it's been
>> propagated in the department.  Thanks again!
>>
>> On Thu, Sep 10, 2009 at 1:03 PM, Leslie Chen <lchen <@t> mednet.ucla.edu>
>> wrote:
>>
>>> Hello,
>>> Did anyone ever use Permount straight without cutting it with some
>>> solvent?  Journal articles mention using Permount to coverslip, and
>>> the post-doc I work with thinks that it isn't diluted.
>>>
>>> I think maybe people just universally "know" that it should be
>>> diluted so it isn't mentioned in the Materials and Methods.  Any
>>> thoughts?
>>>
>>> Leslie
>>>
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>
>
>
> Merced M Leiker
> Research Technician II
> Cardiovascular Medicine
> 348 Biomedical Research Building
> State University of New York at Buffalo
> 3435 Main St, Buffalo, NY 14214  USA
> leiker <@t> buffalo.edu
> 716-829-6118 (Ph)
> 716-829-2665 (Fx)
>
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Merced M Leiker
Research Technician II
Cardiovascular Medicine
348 Biomedical Research Building
State University of New York at Buffalo
3435 Main St, Buffalo, NY 14214  USA
leiker <@t> buffalo.edu
716-829-6118 (Ph)
716-829-2665 (Fx)

No trees were harmed in the sending of this email.
However, many electrons were severely inconvenienced.