[Histonet] Re: Permount question

Tony Henwood AnthonyH <@t> chw.edu.au
Sun Sep 13 18:15:44 CDT 2009

It is possible that the mountant was too thick and so xylene was added
to thin it?

It would save the lab money in mountant costs but there is a possibility
that with less mountant on the slide you may have shrinkage of the
mountant causing eventual cracking of the mountant and possible
detachment of the coverslip.

I would review a sample of slides one and two years old and record the
quality (another audit!)


Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC)
Laboratory Manager & Senior Scientist
Tel: 612 9845 3306
Fax: 612 9845 3318
the children's hospital at westmead 
Cnr Hawkesbury Road and Hainsworth Street, Westmead 
Locked Bag 4001, Westmead NSW 2145, AUSTRALIA 

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Merced M
Sent: Saturday, 12 September 2009 12:30 AM
To: Leslie Chen; histonet <@t> lists.utsouthwestern.edu
Subject: Re: [Histonet] Re: Permount question

Just so a stereotype doesn't get started here...I was 1st taught in the 
(straight) use of Permount by such an Asian technician (who couldn't
English, but was such a wonderful teacher I learned more from watching
than anything!)  :-)

--On Thursday, September 10, 2009 1:52 PM -0700 Leslie Chen 
<lchen <@t> mednet.ucla.edu> wrote:

> Thanks for all the replies, I guess these weird Asian technicians who 
> are highly looked up to taught me something wrong and it's been 
> propagated in the department.  Thanks again!
> On Thu, Sep 10, 2009 at 1:03 PM, Leslie Chen <lchen <@t> mednet.ucla.edu>
> wrote:
>> Hello,
>> Did anyone ever use Permount straight without cutting it with some 
>> solvent?  Journal articles mention using Permount to coverslip, and 
>> the post-doc I work with thinks that it isn't diluted.
>> I think maybe people just universally "know" that it should be 
>> diluted so it isn't mentioned in the Materials and Methods.  Any 
>> thoughts?
>> Leslie
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Merced M Leiker
Research Technician II
Cardiovascular Medicine
348 Biomedical Research Building
State University of New York at Buffalo
3435 Main St, Buffalo, NY 14214  USA
leiker <@t> buffalo.edu
716-829-6118 (Ph)
716-829-2665 (Fx)

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