[Histonet] Re: Permount question

[Tony Henwood]

It is possible that the mountant was too thick and so xylene was added
to thin it?

It would save the lab money in mountant costs but there is a possibility
that with less mountant on the slide you may have shrinkage of the
mountant causing eventual cracking of the mountant and possible
detachment of the coverslip.

I would review a sample of slides one and two years old and record the
quality (another audit!)

Regards

Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC)
Laboratory Manager & Senior Scientist
Tel: 612 9845 3306
Fax: 612 9845 3318
the children's hospital at westmead 
Cnr Hawkesbury Road and Hainsworth Street, Westmead 
Locked Bag 4001, Westmead NSW 2145, AUSTRALIA 




-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Merced M
Leiker
Sent: Saturday, 12 September 2009 12:30 AM
To: Leslie Chen; histonet <@t> lists.utsouthwestern.edu
Subject: Re: [Histonet] Re: Permount question


Just so a stereotype doesn't get started here...I was 1st taught in the 
(straight) use of Permount by such an Asian technician (who couldn't
speak 
English, but was such a wonderful teacher I learned more from watching
her 
than anything!)  :-)


--On Thursday, September 10, 2009 1:52 PM -0700 Leslie Chen 
<lchen <@t> mednet.ucla.edu> wrote:

> Thanks for all the replies, I guess these weird Asian technicians who 
> are highly looked up to taught me something wrong and it's been 
> propagated in the department.  Thanks again!
>
> On Thu, Sep 10, 2009 at 1:03 PM, Leslie Chen <lchen <@t> mednet.ucla.edu>
> wrote:
>
>> Hello,
>> Did anyone ever use Permount straight without cutting it with some 
>> solvent?  Journal articles mention using Permount to coverslip, and 
>> the post-doc I work with thinks that it isn't diluted.
>>
>> I think maybe people just universally "know" that it should be 
>> diluted so it isn't mentioned in the Materials and Methods.  Any 
>> thoughts?
>>
>> Leslie
>>
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Merced M Leiker
Research Technician II
Cardiovascular Medicine
348 Biomedical Research Building
State University of New York at Buffalo
3435 Main St, Buffalo, NY 14214  USA
leiker <@t> buffalo.edu
716-829-6118 (Ph)
716-829-2665 (Fx)

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