[Histonet] Permount question

[Rene J Buesa]

I always diluted Permount to give it the fluidity I desired for manual coverslipping and faster drying.
René J.

--- On Thu, 9/10/09, Leslie Chen <lchen <@t> mednet.ucla.edu> wrote:


From: Leslie Chen <lchen <@t> mednet.ucla.edu>
Subject: [Histonet] Permount question
To: histonet <@t> lists.utsouthwestern.edu
Date: Thursday, September 10, 2009, 4:03 PM


Hello,
Did anyone ever use Permount straight without cutting it with some solvent?
Journal articles mention using Permount to coverslip, and the post-doc I
work with thinks that it isn't diluted.

I think maybe people just universally "know" that it should be diluted so it
isn't mentioned in the Materials and Methods.  Any thoughts?

Leslie
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