[Histonet] Re: Permount question

Merced M Leiker leiker <@t> buffalo.edu
Fri Sep 11 09:30:19 CDT 2009


Just so a stereotype doesn't get started here...I was 1st taught in the 
(straight) use of Permount by such an Asian technician (who couldn't speak 
English, but was such a wonderful teacher I learned more from watching her 
than anything!)  :-)


--On Thursday, September 10, 2009 1:52 PM -0700 Leslie Chen 
<lchen <@t> mednet.ucla.edu> wrote:

> Thanks for all the replies, I guess these weird Asian technicians who are
> highly looked up to taught me something wrong and it's been propagated in
> the department.  Thanks again!
>
> On Thu, Sep 10, 2009 at 1:03 PM, Leslie Chen <lchen <@t> mednet.ucla.edu>
> wrote:
>
>> Hello,
>> Did anyone ever use Permount straight without cutting it with some
>> solvent?  Journal articles mention using Permount to coverslip, and the
>> post-doc I work with thinks that it isn't diluted.
>>
>> I think maybe people just universally "know" that it should be diluted so
>> it isn't mentioned in the Materials and Methods.  Any thoughts?
>>
>> Leslie
>>
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Merced M Leiker
Research Technician II
Cardiovascular Medicine
348 Biomedical Research Building
State University of New York at Buffalo
3435 Main St, Buffalo, NY 14214  USA
leiker <@t> buffalo.edu
716-829-6118 (Ph)
716-829-2665 (Fx)

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