[Histonet] H&E preparation

[Lynette Pavelich]

Ah, the good 'ol days!  Remember adding the oxidizinng agent (mecuric
oxide) too soon when it was still bubbling and having a volcano of
hematoxylin?!!!  LOLj
Was remembering tho', that every day, before using, we would "top off"
(add...oh, about 30ml) of fresh to the filtered used batch.  This really
kept the quality good.  Worked for 30 yrs until we started purchasing
it.  Gone are the morning coffee and donuts sitting next to our
microtomes too!! Sigh................  Ya, ya....I know!!!  

>>> "Kemlo Rogerson" <Kemlo.Rogerson <@t> waht.swest.nhs.uk> 09/02/09 3:53 AM
>>>
Ripening in the sun (we do get some in the UK) used to give Haematoxylin
that was stable; if you forgot to make any up then you hastely added
oxidising agent to a new batch or 'jumped started' a batch that was as
yet still immature. That batch then never seemed to last as long as you
got muddy staining. They looked quite pretty, lines of 5 litres flasks
with their contents ripening in the sun with cotton wool balls sticking
out of the top, next to the cigarette ashtrays. How things have
changed.............. 


 





Kemlo Rogerson	
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-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Aazath
Raj
Sent: 01 September 2009 16:49
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] H&E preparation


Dear All,
          We are preparing harris heamtoxylin for H&E in our laboratory.
some time we get intense nuclear staining some time light weak.Does
anybody know how to control a consistency in the strength .


Aazath
Technical Officer
Apollo Hospitals
India

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