[Histonet] H&E preparation

Kemlo Rogerson Kemlo.Rogerson <@t> waht.swest.nhs.uk
Wed Sep 2 02:53:46 CDT 2009

Ripening in the sun (we do get some in the UK) used to give Haematoxylin
that was stable; if you forgot to make any up then you hastely added
oxidising agent to a new batch or 'jumped started' a batch that was as
yet still immature. That batch then never seemed to last as long as you
got muddy staining. They looked quite pretty, lines of 5 litres flasks
with their contents ripening in the sun with cotton wool balls sticking
out of the top, next to the cigarette ashtrays. How things have


Kemlo Rogerson	
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-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Aazath
Sent: 01 September 2009 16:49
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] H&E preparation

Dear All,
          We are preparing harris heamtoxylin for H&E in our laboratory.
some time we get intense nuclear staining some time light weak.Does
anybody know how to control a consistency in the strength .

Technical Officer
Apollo Hospitals

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