[Histonet] Mast cell staining HELP!

[Kim O'Sullivan]

Hi,

Can anyone out there recommend the best way to stain mouse mast cells in the kidney(toluidine blue/or berberine sulfate) with a protocol that produces cconsistent results?

Secondly, I have mouse kidney sections fixed in FFPE, PLP and methyl Carnoys. Does anyone know of a company that supplies antibodies  which will work on any of these fixatives,for the staining of the IgE receptor, mast cell trytptase and chymase.

Any advice would be appreciated!


Kim O'Sullivan
Monash University
Melbourne
Australia