[Histonet] Endogenous biotin blocking

[Sebree Linda A]

We use biotin blocking only with certain tissues, i.e. liver, kidney,
GI, or diseases, i.e. oncocytomas.

Linda A. Sebree
University of Wisconsin Hospital & Clinics
IHC/ISH Laboratory
DB1-223 VAH
600 Highland Ave.
Madison, WI 53792
(608)265-6596



-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Sally
Price
Sent: Tuesday, September 01, 2009 7:32 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Endogenous biotin blocking


I recently had a discussion with one of my coworkers about
the need/requirement for blocking of endoegnous biotin whenever an
avidin-biotin detection system is used, and I was hoping that the IHC
experts on the histonet might be able to provide us with some feeback.
Its been my understanding that blocking is only necessary when one is
certain that background staining is caused by endogenous biotin, but
maybe I'm off-base here. I look forward to eveyone's input. Sally
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