[Histonet] Endogenous biotin blocking

[Sally Price]

I recently had a discussion with one of my coworkers about
the need/requirement for blocking of endoegnous biotin whenever an
avidin-biotin detection system is used, and I was hoping that the IHC
experts on the histonet might be able to provide us with some feeback.  Its
been my understanding that blocking is only necessary when one is certain
that background staining is caused by endogenous biotin, but maybe I'm
off-base here.
I look forward to eveyone's input.
Sally