[Histonet] List of Resins and plastic embedding mediums

[RICKY MATHIS]

Hello all histo-netters,
I want to know if there is a list of resins and plastic embedding mediums that would also give there properties, stainability, etc.  I am a novice in this area and have received many requests lately for embedding without heat and/or without xylene (or other solvents).  My 33 years have all been with paraffin and frozen sections.  Any information would be greatly appreciated.
Cathy




________________________________
From: Young Kwun <kwuny <@t> email.cs.nsw.gov.au>
To: "Bauer, Karen L." <Bauer.Karen <@t> mayo.edu>
Cc: Histonet <histonet <@t> pathology.swmed..edu>
Sent: Mon, November 9, 2009 9:17:59 PM
Subject: RE: [Histonet] IgG4 antibody


Dear Karen,
We are using Invitrogen's monoclonal Anti-human IgG4 (Cat No. 05-3800) with
a working dilution around 1/700.
Although we are using BondMax stainer with 30 min, Epitope Retrieval
solution 2 (alkaline) pretreatment from Leica, Ventana would give a good
staining as well.

Young



Sun Young Kwun
Senior Hospital Scientist
Immunohistochemistry
Department of Anatomical Pathology
Concord Hospital, Concord NSW 
Australia

61-2-97676075 (tel)
61-2-9767-8427 (fax)
0408868288 (mob)
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Bauer, Karen
L.
Sent: Tuesday, 10 November 2009 8:18 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] IgG4 antibody

Hello,

I'm trying to find an IgG4 antibody to use on our Ventana Ultra IP
stainer.  Is anyone out there using this antibody on their XT or Ultra?
If so, could you please share with me the vendor you go through and what
you are using for a protocol?

Thanks much,

Karen

Karen L. Bauer HTL (ASCP)
Histology Section Chief
Department of Pathology - Luther Hospital
Luther Midelfort - Mayo Health System
715-838-3205
bauer.karen <@t> mayo.edu

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