[Histonet] IgG4 antibody

[Young Kwun]

Dear Karen,
We are using Invitrogen's monoclonal Anti-human IgG4 (Cat No. 05-3800) with
a working dilution around 1/700.
Although we are using BondMax stainer with 30 min, Epitope Retrieval
solution 2 (alkaline) pretreatment from Leica, Ventana would give a good
staining as well.

Young



Sun Young Kwun
Senior Hospital Scientist
Immunohistochemistry
Department of Anatomical Pathology
Concord Hospital, Concord NSW 
Australia
 
61-2-97676075 (tel)
61-2-9767-8427 (fax)
0408868288 (mob)
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Bauer, Karen
L.
Sent: Tuesday, 10 November 2009 8:18 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] IgG4 antibody

Hello,
 
I'm trying to find an IgG4 antibody to use on our Ventana Ultra IP
stainer.  Is anyone out there using this antibody on their XT or Ultra?
If so, could you please share with me the vendor you go through and what
you are using for a protocol?
 
Thanks much,
 
Karen
 
Karen L. Bauer HTL (ASCP)
Histology Section Chief
Department of Pathology - Luther Hospital
Luther Midelfort - Mayo Health System
715-838-3205
bauer.karen <@t> mayo.edu
 
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