[Histonet] RE: CD11c for murine tissu

[Patsy Ruegg]

An alternative to cd11c for macrophages is rat anti mouse f4/80 which I use
from Serotec, it works in frozen and ffpe tissue.
Patsy

Patsy Ruegg, HT(ASCP)QIHC
IHCtech
12635 Montview Blvd. Ste.215
Aurora, CO 80045
720-859-4060
fax 720-859-4110
www.ihctech.net 
www.ihcrg.org


-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of gayle callis
Sent: Saturday, May 30, 2009 8:45 AM
To: 'Histonet'
Subject: [Histonet] RE: CD11c for murine tissu

You wrote:  Hi all - I'm looking for a good antibody to use on mouse tissue
(frozen) 

to look for CD11c - does anyone have a suggestion for a good antibody 

source? Thanks in advance - Melissa

 

We us CD11c, HL3 clone, Armenian Hamster anti Mouse.  Negative control is
Armenian Hamster IgG1 from BD Pharmingen/Invitrogen.  Tissues are fresh
frozen sections fixed with our favorite acetone/absolute ethanol fixative,
although you can use 4C acetone for 10 min on overnight air dried sections.
I will be happy to send the other fixative recipe if you want it. 

 

Gayle M. Callis

HTL(ASCP)HT,MT

Bozeman MT 

 

 

 

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